I can’t believe that after this week, it’s only two weeks until I’ll be back home in Atlanta! I hate to say that it feels like everything is winding up, but with poster preparations and presentations in the near future I fear time has caught up with me. I remember saying, “Hey, even if I hate it, it’s literally only two months, summer’s gonna fly by,” but you never actually think about how it feels when the end is actually approaching. This week I’ve been working on preparing a small presentation for Saturday, where along with the other REU’s, I will be talking to some Oregon high schoolers about what I have been working on this summer as well as how I got from high school to where I am now. I’m super excited to share as I remember being in high school and having absolutely zero clue as to what my future career would look like.
At the start of this week, I went on a series of plankton tows, to try and collect some microorganisms to use in some final experiments with my larvae. However, finding the fast-swimming microorganisms proved to be a little more difficult than I thought, so I wasn’t able to complete any trials of my larvae tethered to see if they would react to other organisms as stimuli.
A little later this week, George, Maureen, and I injected some eggs with the protein encoding mRNA to make both the microtubules of the spindle apparatus as well as the chromosomes fluoresce under the microscope. However, due to some timing issues, we didn’t really get to see anything of use :(. Don’t worry though, I’m attaching some videos we took over the last few weeks of how these caffeine treatments affect both first and second meiosis. We treated eggs with caffeine both prior to meiosis I and meiosis II, to see if we would get a breakdown of the spindle apparatus. Not only did we see that, but after the breakdown of the spindle, when we washed the eggs with filtered sea water, we saw it come back. Eggs treated before either meiosis I or II, once washed out, were even able to continue on to expel a polar body (the end of meiosis).
In addition to my videos, I just wanted to include a couple pictures I took throughout the week; me at the docks, along with some pretty pics of the sky. Well, until next week…:)
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