This past week has been much the same work so far. I am still working on creating a timeline from Meiosis 2 to the first few stages after the mitotic spindle forms. Of course, pro-nuclear fusion occurs between these two steps. I have had a set back this week, as two of my broods of embryos had trouble with the staining of microtubules. This upcoming week, I am going to replace the tubes of anti-bodies with new mixtures, as well as expirementing with differing anti-bodies. I have been using an anti-mouse anti-body, but we have ones such as anti-rat in the lab. They would stain for the same thing, microtubules, but would help us narrow down where the problem is occurring. When done correctly, we can see as above, the microtubules attaching to the condensed DNA, pulling half in each direction in preparation for the first cleavage. This is only at one specific depth in the embryo. With our confocal microscope, we are able to scan the embryo at many depths and the program we have puts all the images together. There we can see the embryo as a whole, instead of in slices. My plan is also to start staining very young, pre-meiosis 2, embryos to see of there is a consistent pattern in regard to where the sperm enters the cell. I Outside the lab this week, I have been able to go fly fishing a lot this past weekend at the local rivers. It was fun to catch a bunch of trout and get into the quiet of nature. I drove inland to the rivers, which got really hot really fast. I had to take a break mid-day to swim in the river. Even as it was ice-cold, it was very refreshing after being in the 90 degree sun.
Also, the REUs and the OIMB students got together for a fun relaxing evening at the OIMB beach down the street. I call it our "secret" beach because I didn't even know it existed until this week! It has been nice to grow our little community and get along with the students here. I can't believe it is almost half way through the summer!
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AuthorHello I am Gina Magro, currently attending University of Wisconsin-Stevens Point. I am studying Biology with a minor in French as a senior this fall. Archives
August 2022
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