This week has been full of adventures inside the lab and out! For the first 3 days of this week, Sherlyn and I were alone in lab, as both Professor George and Erin being out of town. Monday morning as I went to look at my slides under the confocal microscope, the fluorescent tagging didn't appear. I had to look at every step of the fixation and staining process to see where something could have gone wrong. Wednesday, I was able to fix the problem, as creating a new anti-body mixture using DM1A anti-mouse primary anti-body serum. Having the experience to trouble shoot and learn of my mistakes on my own has a great experience. It has given me the confidence to work on a lab alone, as even as things may not go perfect, I can figure it out and get back on track without needing a professor all time time. It makes me excited for the future, for when I am working in my own lab, creating my ow experiments. This week, I have been able to create document the sequence of events between two and four hours after meiosis two. In this time period, we are seeing the mitotic spindle form and separate the chromosomes in preparation of first cleavage. We see the cortical microtubules arranging on the surface of the cell as if to squeeze the cell causing first cleavage, but then tend to disappear. I am unsure as why that would occur and I am planning on continuing creating this sequence and timeline past four hours to hopefully understand the large picture instead of snapshots of what is occurring. Hopefully, by the time of poster presentations I will have a complete sequential order and hopefully a timeline to the DNA movement and microtubules order of the B. glandula embryo. For the adventures outside of the lab, Sherlyn and I went to the sand dunes just north of North Bend. Seeing two sand dunes, a beach, and swam in the North Fork of the Umpqua River. This REU program is about furthering your academic knowledge, but it also about experiencing a new area. It is amazing to be in Oregon for me as the environment here is incredible. You can stand on the top of a dune and look east to see the rolling hills and forest, then turn west and see the ocean. It was a breath taking landscape that I tried to capture in a photo, but it is never quite the same as seeing it.
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AuthorHello I am Gina Magro, currently attending University of Wisconsin-Stevens Point. I am studying Biology with a minor in French as a senior this fall. Archives
August 2022
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